FastQCFastQC Report
Thu 28 Apr 2016
ERR1041626.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041626.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4608357
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC884021.9182975624501313No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG645831.4014322241093735No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA540301.172435208470177No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG373160.8097462935271724No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC317300.6885317261661803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT282280.6125393497074988No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG262100.5687493395151461No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT260140.5644961968007253No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT248880.5400623259005324No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG241170.5233318512432956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT203240.44102485983616285No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC198490.4307174986660105No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA197830.42928531795605246No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG181390.3936109984534618No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT165430.3589782649217498No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC144700.31399477080443206No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC128990.2799045299658859No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128990.2799045299658859No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT124090.2692716731798339No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC121790.26428074040270755No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT109620.23787219609939075No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA107330.23290296302999092No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC104710.22721763960561214No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG104670.22713084077470563No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT104100.22589395743428733No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT103400.22437497789342276No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC102120.2215974153044133No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC98920.21465350883188955No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG90490.19636065521833487No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG88130.1912395241948486No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT85190.18485981012321745No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT85190.18485981012321745No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG82270.17852349546703955No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT80420.1745090495376118No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT78070.16940961822185216No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT75400.16361579625884018No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA70180.15228854882553589No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC70030.15196305320963632No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG63390.1375544472791496No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC63330.13742424903278977No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT58780.1275508820171701No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA56750.12314584134866288No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT56240.1220391562546044No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG55160.11969558782012764No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG54650.11858890272606919No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT52360.11361966965666939No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51520.1117968942076319No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT48780.10585117429053348No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47500.10307361170152399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG4700.018.8936162
AAGACGG11400.017.2017565
GTATAAC5750.017.0521741
CGGTCCA8800.016.60795410
TTAGCGA957.067048E-515.57894827
ACGGACC12750.015.3803918
TACCTGT36750.015.30340237
GGTTATC5950.015.2352932
TCTAGCG9500.015.18947328
GACGGAC12700.015.1496077
CTAGCGG9650.015.14507829
TACCGTC10750.015.1441877
TACTCCG3550.015.1126765
TAGGACG1601.0990334E-815.031254
CACGCAG5100.014.8725495
TGTACCT34750.014.85323635
TTAGAGT8600.014.8430234
GACGATA750.00410689914.813
CGGACCA12750.014.7999999
ATACCGT11650.014.7682416
Produced by FastQC (version 0.11.5)