FastQCFastQC Report
Thu 28 Apr 2016
ERR1041625.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041625.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548425
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC151942.770479099238729No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG103571.8884988831654281No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA96141.753020011852122No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73671.3433012718238593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65421.1928704927747642No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG59901.0922186260655513No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG54090.9862788895473401No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC53460.9747914482381366No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48240.8796097916761636No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG46400.8460591694397593No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT45670.8327483247481424No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA37650.6865113734785978No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC34980.6378265031681634No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34460.6283448055796144No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29290.5340748507088481No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC25150.4585859506769385No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT24140.44016957651456445No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG23930.43634042941149653No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC23150.422117883028673No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT19650.3582987646442084No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC19390.3535579158499339No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA19030.34699366367324613No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG18080.32967133154032No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT17970.3276655878196654No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC17860.3256598440990108No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA17590.32073665496649495No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC17370.31672516752518576No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT16450.29994985640698363No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG16000.2917445411861239No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT15780.2877330537448147No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG15520.28299220495054017No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC15400.28080412089164425No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG15230.2777043351415417No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT14220.2592879609791676No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT13850.2525413684642385No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT13400.24433605324337876No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG13280.2421479691844828No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA12800.23339563294889912No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12530.22847244381638326No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11870.2164379814924557No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG11130.20294479646259744No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10660.19437480056525505No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT10610.19346309887404842No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT10140.18489310297670603No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9610.17522906504991567No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9610.17522906504991567No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9560.17431736335870904No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT9560.17431736335870904No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG8800.16045949765236814No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT8740.15936545562292018No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC8350.15225418243150843No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG7830.14277248484295937No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT7740.14113142179878743No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC7160.13055568218079044No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA7090.12927929981310116No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT7040.12836759812189452No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT6980.12727355609244656No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6930.12636185440123993No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA6820.1243561106805853No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC6690.12198568628344805No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT6650.12125632493048275No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT5920.10794548023886584No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG5920.10794548023886584No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5730.10448101381228062No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5610.10229292975338468No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5500.1002871860327301No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA250.005493868629.620
CACGACC250.005493868629.610
GACTGTG454.0032337E-628.7777777
TATTAGA358.863457E-426.4285722
CACGCAG1300.025.6153855
CGAACGA809.767973E-1025.43750216
TAACGAA809.767973E-1025.43750213
ATTCCGA809.767973E-1025.4375026
ATAACGA809.767973E-1025.43750212
TCCGATA901.4188117E-1024.6666668
CCGATAA851.9717845E-923.9411759
ACGAACG851.9717845E-923.94117515
ACCACGA400.001929906223.1250028
CGATAAC903.8180588E-922.6111110
GATAACG1005.329639E-1022.211
ACTACAC502.699606E-422.23
ACAACGC603.721715E-521.5833323
GAACGAG957.1249815E-921.42105317
AATTCCG957.1249815E-921.4210535
CGCGCAA450.00382329620.55555521
Produced by FastQC (version 0.11.5)