FastQCFastQC Report
Thu 28 Apr 2016
ERR1041624.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041624.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6818942
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT399500.5858680129556755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT375110.5501000008505719No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT307110.45037778587939303No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC259910.3811588366641042No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG189060.2772570876831039No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188400.27628919559661896No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA157580.23109156816409349No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG106720.1565052173782971No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG88780.130196150663842No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC86700.12714582408825298No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT83120.1218957427706527No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT76580.11230481209548344No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG75150.110207712574766No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT70840.10388708394938687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG4550.017.0769232
AAGACGG23050.016.2125825
GACGGAC22400.015.6093767
CGCAAGA23850.015.4360592
ACGGACC23000.015.3630438
ATAACGC19550.015.0460363
CAAGACG27750.015.04
TATACTG8450.014.8875755
GTACGTA3500.014.813
AGACGGA25000.014.436
CGGACCA25350.014.1577919
GCGCAAG26350.014.1119551
ACTCTAA22750.013.82417610
TAAACGC21800.013.74770628
TACGTAG3650.013.68493214
CGCATCG14500.013.65172413
TAACGCC14550.013.6048114
TAATACT15950.013.5705324
TTCGGGC21050.013.44655535
GTATTAG24650.013.4340771
Produced by FastQC (version 0.11.5)