FastQCFastQC Report
Thu 28 Apr 2016
ERR1041623.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041623.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2353926
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT170490.7242793528768534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT145500.618116287427897No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC144780.6150575676550579No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG106840.4538800285140654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT103920.44147522054644023No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA101500.43119452353217563No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76000.3228648649107916No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG67900.28845426746635194No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG59900.25446849221258444No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC56540.24019446660600205No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT49600.21071180657335872No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG44140.1875165149626624No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA35950.15272357754661786No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC33790.14354741822810063No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31390.13335168565197036No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT29000.12319843529490732No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC26070.11075114510821496No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25680.1090943385645938No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT25010.10624802988709076No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG24540.10425136559093191No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA23920.10161746800876492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC6200.017.9032277
TATCACG2550.017.4117642
ACCGTCG6250.017.1688
AAGACGG8050.017.0062125
ATACCGT7000.016.9142866
CGTCGTA6350.016.89763810
GGACCGT1005.8830574E-616.6500026
TATAACG3000.016.652
TTAGAGT5700.016.5526334
CCGTCGT6600.016.537889
TTATACG803.38445E-416.187535
TAGAGTG6300.016.1507955
ATTAGAG7250.016.0758633
ACGGACC8150.015.6625778
TAACGGC1201.9375002E-615.41666736
CGCAAGA8650.015.3988442
GACGGAC8350.015.2874257
ATAACGC8050.015.1677023
TTGGCGT1101.45307495E-515.1363644
TTAACGG1353.976984E-715.07407535
Produced by FastQC (version 0.11.5)