FastQCFastQC Report
Thu 28 Apr 2016
ERR1041622.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041622.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604236
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA424532.64630640379595No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC389032.425017266786184No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG312531.9481547602721794No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG310041.9326333531974098No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG236081.471603928599034No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT226411.411326014376937No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG197291.2298065870607566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT137730.8585395166297228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT129650.8081728623469364No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC128510.8010666759753553No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC123650.7707718814438773No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT109730.6840016057487801No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC102160.6368140348427538No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT97790.6095736537516925No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC96510.6015947778257065No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA95660.5962963055311064No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC91270.5689312545037015No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT84840.5288498699692564No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC81760.5096506997723527No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG79170.4935059430158655No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC76480.4767378365776606No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74770.46607855702028883No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG73600.4587853657441922No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG73350.4572269915398981No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT71330.4446353279692015No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG71220.44394964331931214No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG66300.4132808389788036No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC65870.4106004353474177No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA61490.38329771928818457No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT60440.37675254763014915No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG54520.33985024647246415No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT54300.3384788771726853No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT48760.3039453048055274No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG47880.298459827606412No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC47330.29503140435696495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44200.27552055931920244No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42300.263676915366567No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT40870.25476301491800457No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG40800.25432667014080224No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA36260.2260265945908208No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA35500.22128913700976663No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT34970.2179853836966631No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT34660.21605299968333835No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT33940.21156488197497125No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG30430.18968530814668166No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA30040.1872542443879828No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC29310.18270379171144396No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG27690.172605526867618No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT26640.16606035520958265No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT26420.1646889859098038No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG26090.16263193196013553No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT25330.15789447437908138No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT23720.14785854450342717No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT23010.14343276176323183No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC21480.13389551163295177No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA21080.13140211290608117No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20820.12978140373361527No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20510.12784901972029053No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT19300.12030648857150694No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT18970.11824943462183868No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18850.1175014150037775No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT18530.115506696022281No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA18240.11369898194529982No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG17970.11201593780466215No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA17940.11182893290014687No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCA17190.10715381028726445No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG16700.10409939684684798No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG502.7027488E-422.1999992
CGAGAGT450.003826458520.5555554
AAAAAGT13000.019.4961531
CTCCTGT6300.019.3809537
AAAAGTA13550.018.7047962
CTGTACG609.2389865E-418.59
GACCGTG500.007036067518.4999987
AAGACGG3650.017.7397255
TCGATGC852.724204E-517.4117642
CGCAAGA3650.017.2328762
GAGCGGC650.001580302817.07692316
TATCACG2200.016.8181822
TTAGAGT1801.0913936E-1116.4444454
TGTACTA904.4477216E-516.4444455
GCAAGAC4200.016.2976193
CAAGACG4100.016.2439044
GATAGTC803.3836084E-416.187526
GTAGTGC803.3836084E-416.18756
TATAACG3100.016.1129042
TACCGTC2800.015.8571427
Produced by FastQC (version 0.11.5)