FastQCFastQC Report
Thu 28 Apr 2016
ERR1041621.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041621.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457619
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA135252.9555153959953584No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC127452.785067927686569No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG73821.6131323218660065No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG67831.4822374071006668No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT63771.393517314621989No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG58981.2888450872887707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54231.1850469495366234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54101.1822061583981434No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC38860.8491780280102007No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG34800.760457935531523No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33290.7274610538461035No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC29470.64398549885385No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC29040.6345890358573398No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT28660.626285184837168No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA25750.5626951678142734No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC22980.5021644643251264No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG22980.5021644643251264No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT22910.5006348075582526No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG22260.4864308518658535No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21900.478564045636217No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20220.4418522832312469No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA17880.3907180427386101No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC17470.3817586245326352No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15740.34395425015132675No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15550.3398023246412409No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG15250.3332466527832105No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG13940.30462021900314457No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13170.2877939945675333No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT12190.26637879983130075No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC12000.26222687432121483No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT11800.2578564264158612No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA11310.24714882904774493No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT9870.21568160412919918No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9830.21480751454812846No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT9690.21174820101438097No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG9340.2040999171800122No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9060.1979812901125172No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT9020.19710720053144645No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8370.18290324483904732No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA8310.18159211046744125No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT8290.1811550656769059No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7920.17306973705200177No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC7900.1726326922614664No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT7580.1656399756129007No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC7480.16345475166022388No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG7270.15886578135960264No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7210.15755464698799657No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT6810.1488137511772894No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT6760.147721139200951No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT6380.1394172881807792No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCT6110.13351718350855188No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG6040.13198752674167813No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC5920.12936525799846596No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG5680.12412072051204168No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA5600.12237254134990024No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT5510.12040583979249114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA5510.12040583979249114No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT5250.11472425751553147No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5190.1134131231439254No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGT5090.11122789919124862No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT5050.11035380961017789No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCA4790.10467222733321825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGT200.001840778637.08
GTGTTAC250.005492930329.59999835
CTCGCAC250.005492930329.59999834
TATACTG250.005492930329.5999985
GACCTTA250.005492930329.59999816
TCGAATT250.005492930329.59999813
TAGACTA358.861206E-426.428575
TTACACC451.3215122E-424.6666684
GTATAAC903.812602E-922.6111131
AACAGCG603.7199818E-521.5833327
TATAACG957.1158865E-921.4210532
ATAACGC1301.8189894E-1221.3461533
TATCACG450.003822341820.5555572
GTACAAC450.003822341820.5555571
CTTGGTA450.003822341820.55555717
CCTTGGT450.003822341820.55555716
TGGTATG450.003822341820.55555719
GGGTAGA555.136595E-420.1818181
ACCAACC555.136595E-420.1818188
CGCAATC555.136595E-420.18181812
Produced by FastQC (version 0.11.5)