FastQCFastQC Report
Thu 28 Apr 2016
ERR1041620.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041620.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2616808
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC737172.8170580340628733No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG529512.0234958009911312No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA499341.9082026652318396No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG332221.269562000727604No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC273701.0459307675610896No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG256760.9811954105918355No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG235830.9012124695430463No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT235580.9002571071320478No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA183110.6997456443116958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT179420.6856444951253589No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC177460.6781544538231311No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT153900.5881211002106382No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT149430.5710392203019862No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC127460.4870819716234436No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG123960.47370689786946535No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC117620.449478907126545No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT111110.42460126994414565No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT110200.42112375076811137No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC106440.40675510010669486No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC105730.4040418708594593No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT101210.38676891846860756No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG95160.36364914812244536No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC94540.3612798493431692No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92320.3527962311335031No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA87710.3351793482746919No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG83840.32039033815243606No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT83060.317409607430121No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC82270.3143906622113659No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG80660.308238128284536No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG75830.2897805265040462No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT73500.28087654883354074No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT73030.2790804675008637No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG71440.2730043625669136No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT65930.251948175028508No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA65510.25034316617803065No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG63290.2418595479683645No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT63070.2410188290466859No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT61070.23337592975869836No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC60860.2325734253334597No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT59340.22676482187458918No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT57090.21816656017560326No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG48890.1868306730948545No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT48320.18465244679777806No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45130.172462022433438No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG44580.17036022512924143No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC42400.16202946490533504No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC40930.15641193392866423No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39260.15003011302319466No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT38360.1465908083436003No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT37800.1444507965429638No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA37700.14406865157856444No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA35900.13719004221937567No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT32800.12534354832299505No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA32530.12431175691911672No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG31140.11899994191396542No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC31000.11846493896380628No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT30190.11536956475217135No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT29580.11303848046933515No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA29420.11242704852629616No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT29410.11238883402985624No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG29300.1119684745690169No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT28480.10883488586094203No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27810.10627451459946623No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT26450.10107734308363472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAG3600.021.5833345
GGACCGT603.7286285E-521.5833326
TATAACG5200.021.3461532
TGACGAG450.003827093220.5555575
GTATAAC5700.019.7982441
AAAAAGT19050.019.7139111
AAAAGTA19400.019.4536082
CGTGCAT701.2199524E-418.510
AAGACGG4400.018.55
ATGCGTG701.2199524E-418.57
ATAACGC7400.017.753
TATCACG4400.017.659092
TAACGGC2250.017.26666636
TTAACGG2250.017.26666635
TAACGCA6450.017.2093034
CGCAAGA4650.017.1075292
AGACTAT2300.016.8913046
TCTCGTC2100.016.73809412
GTATCAC5050.016.485151
TCACGCA4850.016.4020614
Produced by FastQC (version 0.11.5)