FastQCFastQC Report
Thu 28 Apr 2016
ERR1041619.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041619.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504715
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT181841.2084680487667099No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT129210.8587008170982544No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67300.44726077695776273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49040.3259088930461915No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT17560.11669984016906855No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16840.11191488089106576No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16000.10633242840006248No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15370.10214558903181002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43850.028.6043341
GTATCAA52600.023.8108372
CGCAAGA3750.021.7066692
GACGGAC3750.021.2133357
CGTAAGC555.14411E-420.1818182
ACGGACC3950.019.2025328
ACGTAAG500.00703586918.4999981
GTACGAC500.00703586918.4999983
AGACGGA4500.018.0888886
ATCAACG74150.017.4645984
CGTGCGA752.0678018E-417.26666610
CAAGACG5250.017.2666664
ATACCGT2250.017.2666666
TCAACGC75000.017.2173335
GCGCAAG4550.017.0769231
CGCCGTA650.001580241117.07692325
TACCGTC1851.8189894E-1217.07
CGGACCA4500.016.8555569
CAACGCA76750.016.7524436
TATCAAC75050.016.7375093
Produced by FastQC (version 0.11.5)