FastQCFastQC Report
Thu 28 Apr 2016
ERR1041618.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041618.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10045732
Sequences flagged as poor quality0
Sequence length42
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT768770.7652702660194399No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT605920.6031616212735915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT395010.39321176396105334No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148330.14765474531870848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114350.028.4127671
GTATCAA297600.021.2782271
TAGACCG1253.938625E-614.45
ATCAACG454650.013.7499183
TCAACGC459050.013.61420254
TATCAAC467400.013.4672662
AACGCAG465200.013.4458296
CAACGCA467000.013.3862945
TTAACGG20550.013.313868535
TAACGGC20750.013.09879536
ACGCAGA496150.012.5998197
TAGGACC18600.012.4838714
CGCAGAG501200.012.4620918
CAGAGTA524100.011.91070510
AGAGTAC523850.011.9060811
GCAGAGT524150.011.8820949
GTATTAG48350.011.7269911
TCGCTAA4350.011.58620614
GGCACCG24200.011.4545459
CGGGTAT2554.5165507E-911.29411817
Produced by FastQC (version 0.11.5)