FastQCFastQC Report
Thu 28 Apr 2016
ERR1041617.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041617.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10048871
Sequences flagged as poor quality0
Sequence length42
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT696380.6929932725775861No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT541910.5392745115346789No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT354520.3527958513946492No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134630.13397524955788567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129300.025.3085841
GTATCAA311400.020.8959541
AACGCAG471450.013.6341096
TATCAAC474450.013.6123922
ATCAACG474300.013.5787483
TCAACGC479500.013.4089684
CAACGCA485600.013.2442335
ACGCAGA502850.012.7827397
TAACGGC20000.012.7836
CGCAGAG504950.012.7153198
GTATTAG37900.012.6807381
TTAACGG20350.012.47174535
TAGGACC21350.012.3091334
AGAGTAC522850.012.21803711
CAGAGTA528150.012.13973210
GGCACCG21750.012.0827599
GCAGAGT531050.012.0429349
ACCGCCT22500.011.9212
AGGTCGC21050.011.88598635
GCACCGC22000.011.86363610
Produced by FastQC (version 0.11.5)