FastQCFastQC Report
Thu 28 Apr 2016
ERR1041616.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041616.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5107083
Sequences flagged as poor quality0
Sequence length42
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT195850.3834870120575679No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT146610.2870718960314528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT95020.1860553274736283No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGC62090.12157625008248348No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCC51300.10044872973476249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48300.021.9130421
GTATCAA104750.019.3489251
TAACGGC14600.017.01369936
TTAACGG14600.017.01369935
TTTAACG16400.015.47560934
GTATTAG23600.015.1016941
GGCACCG17050.014.8856319
TAGGACC10600.014.6037744
ACCGCCT18450.014.14634112
GCACCGC18150.014.08264510
TATTAGA22800.013.9736842
ATTAGAG20650.013.9467323
ACGAACG1703.5002813E-813.76470729
AGGTCGC13500.013.635
AACGCAG146200.013.5430926
GTACCGT1204.276148E-513.5000016
ATCAACG148050.013.4224923
CGAACGC1755.1184543E-813.371428530
TCAACGC148750.013.3230264
GTGACAC19000.013.07368524
Produced by FastQC (version 0.11.5)