FastQCFastQC Report
Thu 28 Apr 2016
ERR1041615.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041615.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7818607
Sequences flagged as poor quality0
Sequence length42
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT295950.3785201123422625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT215270.2753303753469128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT140360.17952046956702133No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGC103330.13215909176660243No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTA84430.1079859877852922No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCC82740.10582447742929144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64650.021.2436181
GTATCAA152150.020.2773591
TTAACGG23850.017.43396235
TAACGGC24150.017.14285936
TTTAACG26250.015.9085734
GTATTAG38950.014.788191
ATCAACG210200.014.3263563
AACGCAG211100.014.256756
TCAACGC211200.014.2244324
CAACGCA215500.013.9656615
CCGTTAA22000.013.90909116
AGGTCGC23650.013.77589935
TATCAAC220950.013.7270882
TATTAGA36850.013.6770692
ATTAGAG34000.013.6588233
GGCACCG30850.013.5948139
TAGGACC18950.013.5831144
CCGAGGT24200.013.4628132
GCACCGC31250.013.305610
ACGCAGA225900.013.2988057
Produced by FastQC (version 0.11.5)