FastQCFastQC Report
Thu 28 Apr 2016
ERR1041613.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041613.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7924635
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT519680.6557778370865031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT485990.613264838065097No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT413210.5214246460562536No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190330.24017509954717156No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80930.10212457734646455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139900.023.7233731
CTTATAC96300.015.54153637
GTATCAA223150.014.79005052
TCTTATA148650.013.77699437
TATAGTC14850.013.2053875
CTCTTAT228750.013.11781437
TTATAGT19400.011.538664
TATCAAC328350.011.2459271
TCTCTTA329250.011.11966637
ATCAACG332950.011.0905552
GTATTAG12450.010.9959841
TCAACGC341400.010.8485643
CAACGCA344050.010.7596274
TTTATAG23150.010.7084243
GTCTAGT8150.010.6687121
CTAATAC12400.010.5927413
ATAGTCA17800.010.3932586
GTATTAA11100.010.3333331
AACGCAG358850.010.326185
TATACAC42050.010.25089337
Produced by FastQC (version 0.11.5)