FastQCFastQC Report
Thu 28 Apr 2016
ERR1041612.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041612.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3566414
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT142440.3993927794137192No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT133890.37541911847586956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT102150.2864221596258875No Hit
CCCACGCCCTCTGCATCTTTTTTCTTTTCCCCCACGGATCTTT45530.12766324941523896No Hit
GTGCAGAGGGCGTGGGGGAAAAGAAAAAAGATCCGTGGGGGAA40280.11294258041831375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52100.024.8560471
GTATCAA81300.015.8603942
TATACCG2201.8189894E-1215.1363645
TCTTATA53500.014.97289837
CTTATAC32600.014.92484837
TAATACG1551.2123382E-714.322584
CTCTTAT86550.014.04332637
TATACAC13850.014.0252737
TAATACT6850.013.7737244
TTATAGT8500.013.0588244
GTACTAG2753.6379788E-1212.7818171
TTAGACT3050.012.7377044
TATACTG6050.012.537195
TACACCG2901.2732926E-1112.1206895
ATCAACG122150.011.7527642
TATAGTC8200.011.7317075
TATCAAC121850.011.7209691
CCGTATA1901.4360849E-611.6842112
TTATACT6850.011.6131394
ATACCGA2552.935849E-911.6078436
Produced by FastQC (version 0.11.5)