FastQCFastQC Report
Thu 28 Apr 2016
ERR1041610.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041610.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5637764
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT123030.21822481395106286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT113850.20194176272720887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT87800.15573550081202406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47400.025.5253181
TCTTATA87150.016.51520337
CTTATAC52600.015.82699537
GTATCAA76100.015.8015772
CTCTTAT138150.013.51176237
TCGCTAA3600.013.36111114
TACCCCG9450.013.1164035
ATCAACG114800.012.7308372
TATACAC18650.012.6970537
CAACGCA118350.012.2708064
TCAACGC119350.012.2609983
TATCAAC124550.011.7788041
TATAGTC9550.011.6230375
AACGCAG126250.011.590895
CTCGCTA4150.011.59036213
GTATTAG19400.011.538661
ATACCGA2603.983587E-911.3846156
TCTCTTA208750.011.25509137
TCTATAC7300.011.1506853
TCTATCG5350.011.06542131
Produced by FastQC (version 0.11.5)