FastQCFastQC Report
Thu 28 Apr 2016
ERR1041608.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041608.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9235073
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT121530.1315961443943107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT103960.11257084811349082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49900.024.2464921
CTTATAC78150.017.35188737
TCTTATA136500.016.50769237
GTATCAA81950.014.6058572
TAATACG7300.014.1917814
CTCTTAT215500.013.86426937
GTACTAG6100.013.0409831
GTATTAG25600.012.3574231
AATACGC7450.012.1677855
ATCAACG117300.011.9548172
TTAACGG9650.011.88601135
TCAACGC118950.011.7889863
CAACGCA119000.011.7684874
TATACTG15050.011.6777415
CTAATAC25300.011.553363
ATTAGAG17050.011.5014673
CTCTAAT15450.011.4951451
TAATACT30050.011.2662224
CGCCGTA5450.011.20183525
GTATAGA12900.011.1860471
Produced by FastQC (version 0.11.5)