FastQCFastQC Report
Thu 28 Apr 2016
ERR1041607.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041607.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4841721
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT110500.22822463334834864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT102230.21114393001992476No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT78640.16242158521732253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43000.023.1465131
CTTATAC45500.017.32087937
GTATCAA64600.015.4071212
TCTTATA74600.015.37533537
CTCTTAT119250.013.97777837
GCGTTAG1153.5808646E-412.8695651
TATACCG1901.04817445E-712.6578955
ATAGTAC7850.012.2547773
TATACAC16600.012.03614437
GTACTAG3700.012.01
GTATTAG10250.011.7317071
ATACCGA2851.1459633E-1011.684216
AGTACCG4150.011.5903625
TTAACGG3205.456968E-1211.56250135
ATCAACG94650.011.5124152
AACCGTT2257.5622665E-811.5111117
CAACGCA95700.011.4054334
TCAACGC96850.011.3464133
TCTCTTA175000.011.25857237
CTACACT8900.011.2247184
Produced by FastQC (version 0.11.5)