FastQCFastQC Report
Thu 28 Apr 2016
ERR1041606.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041606.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3164419
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38660.12217092616369704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34200.020.5014631
CTTATAC34350.017.93449837
TCTTATA60800.016.43092237
GTATCAA49850.014.0651962
GTATTAA3050.013.950821
CTCTTAT96050.013.73295237
TATACTG4650.013.5268815
CCGTATA1951.0279109E-813.2820522
CGTATAC1551.8903011E-613.1290323
CGTTAAC1307.005369E-512.8076931
TATACAC12350.012.73279337
GTATACG2154.0196028E-812.0465121
TTAGAGT3002.1827873E-1111.7166674
TTAGACC2401.4881152E-811.5625014
TAGACAG3850.011.5324675
GATATAT2452.0179868E-811.326531
ATTAGAG3600.011.3055563
CCCGTAT2301.0248732E-711.260871
CGGTTTA1502.6576064E-411.115
TCTCTTA138500.011.09999937
Produced by FastQC (version 0.11.5)