FastQCFastQC Report
Thu 28 Apr 2016
ERR1041605.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041605.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7709956
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT105310.13658962515479986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT88500.11478664729085354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79410.10299669673860656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53300.021.4502811
CTTATAC65200.019.06748437
TCTTATA119850.016.91781437
GTATTAG17250.014.0492751
GTATCAA82550.013.7377352
GTACTAG5450.013.2385321
CTCTTAT183550.013.12285437
CTAATAC16800.012.8839283
TATACTG13150.012.5209135
TAATACT23800.012.0483194
TTAACGG7450.011.67114135
TATACCG3600.011.3055555
TCTATAC9350.011.2780743
GCGCGAA1150.00522305411.2608713
TACCCCG7250.011.2275865
ATCAACG115650.011.1975792
TATTAGA18700.011.0802142
TCAACGC118000.011.0686453
CAACGCA118050.011.0482854
TAATACG3900.010.9102554
Produced by FastQC (version 0.11.5)