FastQCFastQC Report
Thu 28 Apr 2016
ERR1041599.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041599.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4248954
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16400.024.2530481
TCTTATA60750.018.30205737
CTTATAC32000.017.69062637
TATACTG4900.017.3673465
GTATCAA29200.015.5856161
CTCTTAT100400.014.37251137
GTATAAA4650.013.5268811
ACGTTTA2202.8558134E-1013.45454526
TATTAGA8200.013.0853652
CTACGCT1451.3739245E-512.7586214
TTAGACT3500.012.6857154
TATACAC11100.012.66666737
ATAAGAC3250.012.5230773
CGTAATA1359.9722776E-512.3333342
GTATAGA4800.012.3333341
AATCGTC1807.476119E-712.33333328
ATTAGAG7100.012.2464793
GTATTAG13200.012.1931821
ATTAGAC2951.6370905E-1111.9152553
TAAGATA2951.6370905E-1111.9152554
Produced by FastQC (version 0.11.5)