FastQCFastQC Report
Thu 28 Apr 2016
ERR1041598.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041598.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4034894
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT135330.33539914555376177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108880.2698459984326726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT94990.2354213022696507No Hit
CCCACGCCCTCTGCATCTTTTTTCTTTTCCCCCACGGATCTTT52210.12939621214336733No Hit
GTGCAGAGGGCGTGGGGGAAAAGAAAAAAGATCCGTGGGGGAA45270.11219625596112315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70950.024.4580691
TATACAC15100.018.13245237
TATAGTC7150.016.30075
CGCGAAT803.3851998E-416.187535
TACCGAC1902.7284841E-1115.5789487
GTATCAA112900.015.3374672
TCTTATA67250.015.24014937
TAGGTCG2251.8189894E-1214.821
TCTATAC4150.014.7108433
TATTAGA12650.014.4782612
TATACCG2700.014.3888895
GTACCGT908.280365E-414.3888886
TTATAGT8250.014.3515164
CTTATAC42650.014.31418537
ATTAGAG11450.014.218343
GTATTAG16200.014.1604941
TGCGACG1854.9094524E-914.022
GCGCGAA800.006302237513.87511
TTAGAGG15800.013.8164564
TAACCGG2950.013.7966122
Produced by FastQC (version 0.11.5)