FastQCFastQC Report
Thu 28 Apr 2016
ERR1041597.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041597.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2453473
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT122370.4987623666533114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT103450.42164719155254615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86650.3531728288837905No Hit
CCCACGCCCTCTGCATCTTTTTTCTTTTCCCCCACGGATCTTT43370.1767698279133294No Hit
GTGCAGAGGGCGTGGGGGAAAAGAAAAAAGATCCGTGGGGGAA38860.1583877222207051No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26150.10658360617785481No Hit
TATCAACGCAGAGTACGGGGAGCGGATAACAATTTCACACATA25000.10189637301898168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61750.022.229961
TCCGCGA450.003827026620.55555710
TATACCG1750.017.9714285
TATACAC11750.017.00425537
TATACTG3700.015.55
TACCGAC1954.1836756E-1115.1794877
TCTTATA45750.015.04262337
ATACCGA2109.094947E-1214.97619156
GTATCAA92250.014.9002712
GTATTAG3900.014.7051291
TTATAGT5200.014.2307694
CGTATAC2009.858923E-1013.8750013
CGCGATA800.00630130713.87500114
CTTATAC29500.013.73389837
GTATACT2700.013.7037044
CGCGTTA950.001245778213.63157922
TTACACT3800.013.6315794
CCGTATA2051.4260877E-913.5365852
CCGACCG2750.013.4545459
CGGTTTA1254.8487607E-513.3215
Produced by FastQC (version 0.11.5)