FastQCFastQC Report
Thu 28 Apr 2016
ERR1041596.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041596.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3064286
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT170040.5549090391693203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT144760.47241021236268416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT118610.3870722249816107No Hit
CCCACGCCCTCTGCATCTTTTTTCTTTTCCCCCACGGATCTTT46600.15207457789514425No Hit
GTGCAGAGGGCGTGGGGGAAAAGAAAAAAGATCCGTGGGGGAA41300.13477854221179095No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38790.12658740078439154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79550.022.5348831
TATACCG1451.546141E-1017.8620685
AATCGAC904.4498483E-516.4444456
CTTATAC38650.015.41267737
TCTTATA63250.014.91699637
GTATCAA122600.014.5615832
TACCGAC1803.3378456E-914.3888887
TATACAC14800.014.12537
ATACCGA2009.858923E-1013.8750016
TGGACCG1356.575052E-613.7037035
ATTTCGG3400.013.05882429
TATAGTC4700.012.9893625
CTCTTAT97650.012.95852637
TTAACGG2305.7661964E-1012.86956435
TACACCG2052.0691004E-812.6341465
TTATAGT6450.012.6201554
GTATTAG5900.012.5423731
CCGTCGA1205.125765E-412.3333349
CGCTATA3450.012.3333342
TTTCGGT3600.012.33333330
Produced by FastQC (version 0.11.5)