FastQCFastQC Report
Thu 28 Apr 2016
ERR1041595.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041595.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614109
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46660.2890758926441771No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41160.25500136607874685No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG37600.23294585433821383No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32630.20215487305999782No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG28840.1786744265721832No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG20830.1290495251559839No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT19170.1187652135016904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG500.007036084318.523
TCTAACG752.0679188E-417.2666662
GTACTAG1554.0199666E-1016.7096791
GTATACT1708.54925E-1116.323534
ATCGTCA1251.6588092E-716.27999910
CTTATAC15750.015.85714237
GACTTAG855.3658197E-415.2352941
TCTTATA24650.014.86004237
CTCTTAT40750.014.57300637
CGTACCT3750.014.30666733
AGAACCG1304.4463522E-614.2307695
GTATACG1051.6562857E-414.0952381
GTCTAGT1458.915613E-714.0344841
CGTCATT2009.840733E-1013.87500126
ATACCGT1753.5679477E-813.7428576
TACTGTG4050.013.7037047
CCGTACC3950.013.58227832
CGTGAAA1501.3063182E-613.56666710
TTAGGGT2051.4242687E-913.5365864
TTAGACT1652.596571E-713.4545454
Produced by FastQC (version 0.11.5)