FastQCFastQC Report
Thu 28 Apr 2016
ERR1041593.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041593.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038857
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25040.1228139099505262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24380.11957680210039252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.020.8981481
CGAACTA1606.311893E-1016.187524
CTTATAC18350.015.62670337
TCTTATA29650.015.34907237
GGTAATA2201.8189894E-1215.1363642
CTCTTAT49000.014.46020437
GTATCAA31050.014.4186782
TATACAC7800.014.23076837
TATACTG3150.013.5079375
ATTAGAG2750.013.4545453
ATCTAGT4250.013.0588231
CGCGTTT850.00940799613.05882324
GTATTAG4150.012.9277111
TTATAGT2704.3655746E-1112.3333334
TAAGACT3200.012.1406264
GCCGTCA1705.3136246E-611.97058813
GTAATAA2808.367351E-1111.8928583
TATACCG1257.2229054E-411.8400015
TACCGAC1257.2229054E-411.8400017
CGATCAC1100.003746752211.77272729
Produced by FastQC (version 0.11.5)