FastQCFastQC Report
Thu 28 Apr 2016
ERR1041592.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041592.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418550
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT76570.3165946538215046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66150.2735109879886709No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50280.20789315912426867No Hit
CCCACGCCCTCTGCATCTTTTTTCTTTTCCCCCACGGATCTTT25620.10593123979243761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43650.023.2680421
TATACCG1550.020.2903235
GTACTAG2450.017.3673461
ATACCGA1901.8189894E-1216.5526316
TCTTATA37900.015.71767837
GTATCAA65050.015.6133742
TACCGAC1954.1836756E-1115.1794877
GTATTAG5150.015.0873781
CAATGCG2006.184564E-1114.819
TATACAC7750.014.5612937
TGCGACG2059.276846E-1114.43902422
CTTATAC23500.014.17021337
ATTAGAG4000.013.8750013
TACTAGG3400.013.60294152
GAACCGT1254.8487054E-513.326
ACCGGTT850.009408656513.05882413
CCGACCG2551.6370905E-1113.0588249
TAGTACT3850.012.9740264
CTCTTAT60050.012.75437137
GTTCGGT1602.6982998E-612.7187511
Produced by FastQC (version 0.11.5)