FastQCFastQC Report
Thu 28 Apr 2016
ERR1041591.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041591.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4244591
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47520.11195424953782354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48250.028.1430051
TCTTATA62100.016.41465437
GTATCAA83150.016.286232
CTTATAC35750.016.19720337
TCGCTAA2600.014.942306514
TATACAC13900.014.90647537
TATACTG5000.013.6900015
GAACCGT1652.5999543E-713.4545466
TAACCGG3600.013.36111122
CGCTATA5400.013.3611112
CTCTTAT98650.013.239737537
CGATACA1755.3146323E-712.68571427
TAATACG2358.112693E-1012.5957454
TCGATAC2653.274181E-1112.56603826
GTATAAA7600.012.4144741
TCGCTAT6050.012.2314041
ATCTAGT8650.012.1907511
GCGTAGA1257.2257366E-411.8400011
TCGTAGC4850.011.82474214
GTATTAG9900.011.7727261
Produced by FastQC (version 0.11.5)