FastQCFastQC Report
Thu 28 Apr 2016
ERR1041588.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041588.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988339
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13790.13952702463426012No Hit
CCCACGCCCTCTGCATCTTTTTTCTTTTCCCCCACGGATCTTT13410.13568219001779755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13050.1320397151179909No Hit
GTGCAGAGGGCGTGGGGGAAAAGAAAAAAGATCCGTGGGGGAA12930.13082555681805536No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG12700.12849842007651222No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG555.142392E-420.18181826
TACGGTG609.235567E-418.528
AGACGTT609.235567E-418.56
TCTACGC500.007034201618.53
TATAGAG801.6162969E-518.57
TATACTG1054.7955837E-717.6190475
GGTATCA22500.017.348891
TATACAC3850.017.298737
TCGATAC1005.8761125E-616.65000226
CTCGCTA1251.6568993E-716.27999913
TCTTATA15500.016.11290437
AATCGTC700.00259214815.85714328
ATCTAGT2450.015.8571421
ATAGACG1059.340367E-615.8571423
GGTCGTC957.0580754E-515.57894823
TTAGCGA957.0580754E-515.57894827
ATTACTC2300.015.2826093
CTTATAC9350.014.83957337
GATCGTT750.004103865514.811
ATTAGAG1752.235538E-914.83
Produced by FastQC (version 0.11.5)