FastQCFastQC Report
Thu 28 Apr 2016
ERR1041586.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041586.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359139
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG119230.8772465509414415No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG87500.6437899287710822No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG68350.5028919043600397No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT65640.48295281056610107No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA32400.2383862136249493No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG29870.21977148768448257No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG29610.21785851189613423No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTA28430.2091765448567071No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA26960.1983608740533529No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC25570.18813381118487513No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA25110.18474931555933571No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCT24470.18004045208032438No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG24020.17672953244664452No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCT21550.15855626245733512No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCT20720.1524494551329923No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGCTGTCTCT17790.13089168951814348No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCT16600.12213614648685675No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCC16480.12125323458454212No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC16460.12110608260082302No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCC14250.10484578839986197No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG14210.10455148443242376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAA358.8702334E-426.4285714
ACGTTTA753.7418067E-722.226
TATACCG701.2194053E-418.55
TAGTACT1601.8189894E-1218.54
CTTATAC14100.018.23758737
TTTAGAC752.0676164E-417.2666663
CGATAGA650.001580134817.0769238
TAGAACA1005.8793794E-616.654
TATACAC4700.016.53191637
ATACCGA904.446918E-516.4444456
ATCTTAC1606.2937033E-1016.18751
TCTTATA23050.015.570497537
GTATTGA1557.210474E-915.51612951
GTATTAG3350.015.4626871
CTAGTAC1455.3471013E-815.3103453
ATAGGCG855.3650443E-415.2352943
AATGCGA855.3650443E-415.23529420
GTACTAG1101.4521771E-515.1363641
CCTAGTA1853.0559022E-1015.0000012
ATTAGAG2350.014.9574473
Produced by FastQC (version 0.11.5)