FastQCFastQC Report
Thu 28 Apr 2016
ERR1041585.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041585.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960800
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11250.024.1733321
TATACCG652.6844518E-622.769235
GCGTATG652.6844518E-622.769231
ATACCGA701.2197549E-418.56
TCTTATA30650.018.28874437
CTTATAC16100.017.58074637
GTATCAA16650.016.02
GCGTTAT700.002593369715.8571421
TATACTG2500.015.5400015
GTATTAG4150.015.1566271
TCTGTCG3650.014.698638
TAGGCGA1651.646913E-814.5757574
ATAGTAC1956.730261E-1014.2307683
TGCGTCA2009.840733E-1013.87500110
TACCGAC800.00630071113.8750017
ATTAGAC1102.4577466E-413.4545453
CTAGACT2102.042725E-913.2142854
ATTAGAG3650.013.1780823
CTCTTAT47500.013.12526337
AATCGCC850.00940782713.05882412
Produced by FastQC (version 0.11.5)