FastQCFastQC Report
Thu 28 Apr 2016
ERR1041581.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041581.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12750073
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40700.021.909091
TCTTATA118350.015.49091737
CTTATAC74150.015.26904937
TTAACGG16450.015.18237135
GTATTAG36400.014.2307691
TAACGGC18000.014.08055636
TATTAGA28700.013.9878042
ATTAGAG24350.013.9034913
TTTAACG19000.013.82631634
GTATCAA63050.013.7906422
CTCTTAT186750.013.09611837
TATACTG20000.012.48755
TTAGAGG32250.012.4480624
TCTATAC14350.012.3763073
TAATACT37750.011.81059554
GTATAGG25850.011.52224351
AACGGCC21450.011.38461537
CTAATAC32700.011.145263
ATCAACG81450.011.0386742
TCAACGC81300.011.013533
Produced by FastQC (version 0.11.5)