FastQCFastQC Report
Thu 28 Apr 2016
ERR1041578.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041578.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5225135
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53760.10288729382111658No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53030.10149020073165574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38800.026.1288661
GTATCAA58850.017.1639752
CTTATAC42650.016.56975437
TCTTATA68450.015.16216237
CTCTTAT111250.013.90202237
GTATTAG14000.013.8751
TCACGTA9100.013.41758225
CTAACGC1951.0284566E-813.282053
TATACTG6600.013.1742425
TAACCGG4950.013.08080922
TCGCTAA2859.094947E-1212.33333414
AATCGTC2756.184564E-1112.10909128
ATTAGAG10700.012.1028033
TAATACT16400.012.0701234
TCTATAC5550.012.03
ATCAACG85400.011.9145194
GTACTAG2654.2564352E-1011.8679251
GTACCGT1100.00374809511.7727276
CAACGCA86250.011.7113046
TCAACGC86650.011.6999435
Produced by FastQC (version 0.11.5)