FastQCFastQC Report
Thu 28 Apr 2016
ERR1041577.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041577.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2947241
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47430.16093017164188472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41020.139181017093614No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30540.10362233695853172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.023.096971
TCTTATA31600.015.1044337
GTATCAA38650.014.6468312
TAGACCG908.2794274E-414.3888895
CTCTTAT53500.014.1775737
ATACCGA1458.922816E-714.0344836
GTCTTAC1854.9076334E-914.01
ATCGTAT800.006301705313.8758
GTAATAC2700.013.7037043
CTTATAC21200.013.61320837
CGCTAGA1153.580125E-412.8695651
TACCCGC3001.8189894E-1212.33333311
GTATTAG9750.012.3333331
TATACAC8050.011.95031137
ATTAGAC1552.7230695E-511.9354843
TCTCTTA83500.011.89760637
CTAGTAC2502.1500455E-911.843
ATTAGAG6200.011.6370973
TCGTTAC1603.7673337E-511.562513
TAGGACG2257.557901E-811.5111114
Produced by FastQC (version 0.11.5)