FastQCFastQC Report
Thu 28 Apr 2016
ERR1041576.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041576.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8178696
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT126540.15471904078596393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT101340.12390728301920012No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT96110.11751262059379639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71350.026.7841641
GTATCAA116900.016.252782
TCTTATA107500.015.95302337
CTTATAC66950.015.778192537
CTCTTAT172750.013.42923337
TATACAC21600.012.9328737
TATTAGA17550.012.7549862
ATTAGAG15150.012.4554463
TAATACT26700.012.3333334
ATACCGA2704.3655746E-1112.3333336
GTATTAG27250.012.2880741
GTATTAA6950.012.2446041
ATCAACG175000.012.1782862
CTCGCTA4800.011.94791613
CAACGCA178350.011.9184194
TCAACGC179850.011.8601613
TTAACGG11100.011.66666735
AACGCAG184750.011.5856565
TATCAAC185100.011.4937871
TCGCTAA4850.011.44329914
Produced by FastQC (version 0.11.5)