FastQCFastQC Report
Thu 28 Apr 2016
ERR1041574.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041574.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11319327
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT186880.1650981546871117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT165390.1461129270317926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT137780.12172101751279028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70300.023.51
CTTATAC76500.017.62941237
TCTTATA137300.015.63000737
GTATCAA107450.015.3750582
CTCTTAT213650.013.2742837
CTAGACT10800.012.5046294
ATCAACG159300.012.28688052
GTATTAG17550.012.2279211
TCAACGC161800.012.15420153
TATCAAC163850.011.9569731
CAACGCA164650.011.8539324
AACGCAG171600.011.43851955
GTATAGG13100.011.297711
TATACTG18900.011.2566145
TAATACT19050.010.9737534
TAGACCG2901.899025E-910.8448285
TTTCGCG2401.8469837E-710.79166728
TCTCTTA307900.010.77914937
TATACCG3950.010.7721525
CTACACT16450.010.6838914
Produced by FastQC (version 0.11.5)