FastQCFastQC Report
Thu 28 Apr 2016
ERR1041573.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041573.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69013
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTCAGAGAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAA750.10867517714053875No Hit
TCAGAGAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAACCT730.10577717241679105No Hit
CATGTGGACCAGGTTGGCCTCGAACTCGTGCCCTCGAACCCTC710.10287916769304334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCAG250.005461054429.5999985
GGTATCA453.9369534E-628.7777791
CATGTGG358.7848346E-426.428571
TGTGGAC555.076079E-420.1818183
CTGTGGG555.076079E-420.1818189
GGACCAG609.117437E-418.56
GTGGACC500.00696970818.4999984
AGAGGCG500.00696970818.4999988
AGAGAGG500.00696970818.4999986
GTATCAA650.001559681617.0769231
TCTTATA803.3253E-416.187537
CTCTTAT1353.817022E-715.07407537
GTTGGCC850.00928810413.0588233
CCTGACC850.00928810413.05882326
TCTCTTA1951.3907447E-712.33333437
ACCTGTC1250.00954102910.36000137
GTCTCTT2707.8433004E-810.27777837
CTGTCTC5300.09.77358537
CCTGTCT3157.266681E-89.39682537
TGTCTCT3801.5297701E-99.2537
Produced by FastQC (version 0.11.5)