FastQCFastQC Report
Thu 28 Apr 2016
ERR1041571.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041571.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14071056
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT328590.23352191903720657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT300160.21331732316323665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT256360.18218959543619184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96650.027.4868091
CTTATAC87250.016.58108937
GTATCAA162000.016.3645062
TCTTATA141750.014.65643837
CTCTTAT216000.012.89861237
GTACTAG9500.012.2684221
GTATTAG31400.012.2547771
ATCAACG238000.012.0716382
TCAACGC242900.011.86619953
CAACGCA247450.011.6405334
TCTGTCG27550.011.617068
TATCAAC252850.011.4065661
TATACTG21850.011.0915335
AACGCAG261550.011.0695865
CTAATAC30650.010.9249593
TAATACT40850.010.8690334
GTCTCGC27000.010.5518521
TAATACG9600.010.406254
TTAACGG10250.010.28780535
CGCTATA4150.010.2530112
Produced by FastQC (version 0.11.5)