FastQCFastQC Report
Thu 28 Apr 2016
ERR1041568.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041568.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8985527
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT116470.12961955375572295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT102720.11431716804145155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48200.028.0954381
CTTATAC65450.017.41176437
TCTTATA116400.016.27491637
GTATCAA83550.016.1639732
CTCTTAT177200.013.09198737
CTAATAC14500.012.3758623
ATCAACG120250.011.5846162
TCAACGC123400.011.3938413
TATACTG14800.011.255
CTACACT14350.011.2160284
TATCAAC124950.011.1636651
TCTGTCG15250.011.1606568
CAACGCA126050.011.1249514
GTATTAG15050.011.0631241
TAATACT20550.010.9829684
AACGCAG132650.010.6690535
TCTCTTA256500.010.58791437
GTACTAG5250.010.5714281
GTCTAGA8250.010.3151521
GTATTAA15750.010.2190481
Produced by FastQC (version 0.11.5)