FastQCFastQC Report
Thu 28 Apr 2016
ERR1041566.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041566.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2298267
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9200.020.3097841
CTTATAC14000.018.537
TCTTATA26500.018.08113337
TCTGTCG5200.016.0096158
CGCTAGA700.002593550815.8571431
TTTATAC2600.014.9423073
TAGACTA1752.240995E-914.85
CGTAAGA750.004106067614.7999992
GTATAAT2950.014.4237291
TTATACC2353.6379788E-1214.1702124
TAATACG1051.6567676E-414.0952384
ATTAGAC1458.9203604E-714.0344833
AATACGC1854.9058144E-914.05
CTAGTAC1203.3031803E-513.8750013
AGACTAT2009.858923E-1013.8749996
GTATCAA13900.013.8417261
CTCTTAT42650.013.6635437
ATTATAC2457.2759576E-1213.5918383
TGTCGCC6900.013.40579710
TATACTG2900.013.3965515
Produced by FastQC (version 0.11.5)