FastQCFastQC Report
Thu 28 Apr 2016
ERR1041565.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041565.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410252
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT147431.045415996573662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT137370.9740812280358404No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95090.6742766541015365No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49960.3542629260586051No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA21820.1547241202281578No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA14890.1055839665534954No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT14330.10161304504443178No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATCG555.143891E-420.1818185
TGCGACG1303.274181E-1119.92307722
CAATGCG1500.019.73333419
TAGACTG851.2457185E-619.5882365
ACGAGTC1355.638867E-1119.18518426
CGACGAG1552.1827873E-1117.90322724
ACGGACC2900.017.8620688
TCTAGAC1054.799167E-717.6190473
TACACCG1603.45608E-1117.343755
CTAGATA1502.5102054E-1017.2666663
ATGCGAC1502.5102054E-1017.26666621
TAAGGGT1401.869921E-917.1785724
AATGCGA1950.017.07692320
GTACTAG2300.016.8913041
GACGGAC3000.016.657
TACGGGC1151.242608E-616.0869564
TACCGCA3450.016.08695628
CTTCGGG2800.015.85714334
ATACCGA1059.346026E-615.8571426
CCGGTCG2003.6379788E-1215.72499920
Produced by FastQC (version 0.11.5)