FastQCFastQC Report
Thu 28 Apr 2016
ERR1041564.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041564.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247077
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67320.5398223205142906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62640.5022945656122276No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44430.35627310903817483No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19690.1578892081242778No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT15540.12461139127736298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG250.005496520529.65
CTAGCGG1950.021.8205129
TCTAGCG2000.019.42500128
ACCGATA500.007035209318.512
CTACCGG500.007035209318.523
TGTATAC1351.1514203E-917.8148163
CGAACGT953.6068832E-617.5263164
AAGACGG3800.017.0394745
GACGGAC3750.016.7733357
CGACGAG904.4464447E-516.44444524
ACGGACC3950.016.3924058
GAGTTAT803.3828543E-416.18751
CGAGCCG3550.016.1549315
ATAACGC2550.015.9607843
GTAAACG2450.015.85714327
GCGCAAG4100.015.7926841
TAGCGGC2350.015.74468130
TACGACG2350.015.7446815
ACGCTTC2600.015.65384631
CAAGACG4400.015.5568184
Produced by FastQC (version 0.11.5)