FastQCFastQC Report
Thu 28 Apr 2016
ERR1041563.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041563.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250488
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA3600.019.0138930
TTAGTAC1205.180482E-918.53
GGTATCA10500.017.971431
TATTAGA2350.017.3191492
AGGTACG1005.8828264E-616.65000236
CAAGACG5750.016.4086954
CGTTTAT4200.016.29761931
CGTATAC803.3843677E-416.18753
AATAACG4500.016.0333332
GACGGAC5700.015.9035087
TAATACG1059.350975E-615.8571424
TTATACT3050.015.7704934
ACGGACC5800.015.6293118
CTAGCGG4550.015.45054929
TTAGACT1455.3525582E-815.3103454
TAACGAA2550.015.23529413
TAACGCC4500.015.2111114
TCTAGCG4650.015.11827928
ATTAGAG3800.015.0921063
TATACTT2950.015.0508475
Produced by FastQC (version 0.11.5)