FastQCFastQC Report
Thu 28 Apr 2016
ERR1041562.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041562.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3400032
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35820.10535194962870938No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT34700.10205786298481898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG8900.017.2528084
GGTATCA22450.017.2227171
GACGGAC7950.017.2201257
ACGGACC8050.017.0062128
AAGACGG9200.016.6902185
CGCAAGA8950.016.3296072
TCTAGCG5200.016.00961528
GCGCAAG9450.015.6613761
TAACGCC5800.015.6293114
CTAGCGG5500.015.47272729
AGACGGA9650.015.1450786
TATATCG1353.9785118E-715.0740735
GTACCGT1001.09436434E-414.86
TACCGCA9150.014.75956328
GCAAGAC14200.014.721833
ATAACGC6550.014.6870233
CGATGCG6500.014.51538533
CGGACCA9450.014.4867739
ATACCGC9200.014.47826127
GTACTAG2950.014.4237291
Produced by FastQC (version 0.11.5)