FastQCFastQC Report
Thu 28 Apr 2016
ERR1041561.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041561.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1806218
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53530.2963651120739578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52810.29237888228331244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38190.21143627181215113No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT18750.10380806746472462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG1500.020.9666671
CTAGCGG2600.018.529
CTAGTAC1156.410846E-817.6956523
TCTAGCG2750.017.4909128
TGCGACG1201.0421354E-716.95833422
CGTATTA1005.881533E-616.65000215
GCGTTAT2850.016.228071
CGCAATA2850.016.2280736
CCGTATT1059.348936E-615.85714214
TTAACGG1059.348936E-615.85714235
TTCGCGG1403.4764525E-815.85714232
ATGCGAC1557.215931E-915.516129521
TTTAGAC1802.0190782E-1015.4166673
TACCCCG1201.9368927E-615.4166675
TCGAACG2450.015.102043
TAGACTC1353.9754923E-715.0740755
GGTATCA27200.015.031251
CGAGCCG5350.014.86915915
ATGACCG750.004105612614.85
GCATAGG1508.108509E-814.81
Produced by FastQC (version 0.11.5)