FastQCFastQC Report
Thu 28 Apr 2016
ERR1041560.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041560.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168320
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53810.24816447756788665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48350.22298369244391972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36380.1677796635182999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22350.020.8590621
AAGACGG4750.019.084215
TATAGCG500.007036844318.52
ACGGACC4050.018.2716058
AGACGGA4300.018.0697676
CGCAAGA4450.017.8764042
GACGGAC4250.017.4117667
TAACGCC3050.016.9836064
CGAACGA2850.016.87719316
ACGAACG2850.016.87719315
TAACGAA3200.016.187513
ATAACGA3400.015.77941112
CGGACCA4850.015.6391759
CGCAATA2850.015.57894736
GCGCAAG5350.015.5607491
CCGATAA3350.015.4626879
GTAAACG2055.456968E-1215.34146427
TAATCGG855.366876E-415.23529316
GCAAGAC7600.015.0921063
CTAGCGG2950.015.05084729
Produced by FastQC (version 0.11.5)