FastQCFastQC Report
Thu 28 Apr 2016
ERR1041559.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041559.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4974068
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111900.22496676764370732No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT91740.18443656178403672No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT78620.15805976114520348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40600.022.1908871
TAACGCC5500.018.1636374
ATAACGC6400.016.7656253
TCTAGCG4200.015.85714228
CTAGCGG4250.015.67058729
AACGCCG6100.015.4672145
GTATCAA59150.015.3254442
CGCATCG6050.015.28925613
AATAACG6700.015.1865672
TCGTTTA6300.014.9761930
TCTATAC8700.014.459773
TACGACG4900.014.346945
CGTTTAT6850.014.313868531
CGACGGT4800.014.2604167
GTACTAG4450.014.134831
GCATCGC6950.014.10791514
ACGGACC6050.014.0661158
GACGGAC5800.014.0344817
AATCGTC2551.8189894E-1213.78431328
TACCGTC5650.013.7522137
Produced by FastQC (version 0.11.5)