FastQCFastQC Report
Thu 28 Apr 2016
ERR1041558.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041558.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5294768
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT992691.874850796106647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT912211.7228516905745446No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT768771.4519427480108666No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360080.6800675685884632No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA268230.5065944343548197No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA137950.2605402163040949No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110180.20809221480525683No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT92650.17498405973595066No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA85090.16070581373914777No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84860.16027142265723446No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA77030.1454832393034029No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA70410.13298033077181098No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69190.13067616938079252No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA65660.12400921060186207No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62970.11892872360035416No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA58280.1100709228430783No Hit
GAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAA57770.10910770783535748No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT54870.10363060288949393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG8000.018.528
GAACAGT72700.017.5839081
AACAGTG73500.017.3673482
ACGGACC12500.017.1680018
AAGACGG12950.017.05
GACGGAC12650.016.9644267
CTAGCGG8900.016.83707829
CAAGACG14650.016.2901024
ACAGTGG79150.016.150983
CGCAATA9600.015.41666636
CGGACCA14300.015.2657359
AGACGGA14700.015.102046
CGAACGA6900.015.01449216
ACGAACG7150.014.48951115
TAGCGGC10400.014.40865430
TAAACGC8850.014.21468928
TATACTG7000.014.0071435
CGCAAGA15800.013.9335442
TAACGAA7600.013.87513
GCGCAAG16750.013.6955231
Produced by FastQC (version 0.11.5)