FastQCFastQC Report
Thu 28 Apr 2016
ERR1041557.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041557.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9250283
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT328810.3554593951341813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT260550.2816670581862198No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT239220.2586083041999904No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101900.11015879189858299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126550.017.6301861
GACGGAC17350.016.953897
ACGGACC17150.016.935868
AAGACGG19400.016.211345
CGCAAGA19000.014.9947372
TATACTG17650.014.5694055
TTAACGG4350.014.4597735
TAACGCC13500.014.3888884
TCTAGCG13300.014.1879728
CGGACCA21000.014.0071429
AGACGGA22300.013.688346
CGAGCCG24300.013.62757215
ACGAACG8150.013.61963215
ATAACGC16050.013.6012453
CAAGACG23150.013.5853134
CTAGCGG13850.013.49097429
ATACCGC25050.013.21956227
CGAACGA8700.013.18390816
TACCGTC13450.012.9293697
CGCATCG14050.012.90391413
Produced by FastQC (version 0.11.5)