FastQCFastQC Report
Thu 28 Apr 2016
ERR1041556.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041556.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9119065
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT272810.29916444284584004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT202320.22186485127587094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT192800.21142518448985723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99900.020.129631
AAGACGG22750.018.9472525
ACGGACC21650.018.7990748
GACGGAC22000.018.2477287
CAAGACG26350.016.8500964
CGGACCA25200.016.2242079
CGCAAGA26250.016.1390482
TCTAGCG12200.015.92213228
AGACGGA26050.015.7658356
GCGCAAG27100.015.4280441
GCGAAAG25900.015.28571518
TATATCG4250.015.2352935
TAAACGC11150.015.09865528
CTAGCGG13100.014.96946529
GTAAACG11300.014.57079727
CGAAAGC27000.014.45740719
CGCAATA14200.014.33098636
AGCGAAA28800.013.93923717
GTATCAA144100.013.9167242
TATACTG20200.013.7376245
Produced by FastQC (version 0.11.5)