FastQCFastQC Report
Thu 28 Apr 2016
ERR1041555.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041555.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8172623
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT341220.41751589422392293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT240530.29431187514706114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT239230.29272119856746115No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116170.14214530634779066No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT85910.10511924996417919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG19250.019.7012985
GACGGAC19950.018.268177
TCTAGCG13550.018.02213928
CTAGCGG14000.017.57499929
ACGGACC20750.017.5638548
CGCAAGA21000.017.4428582
CAAGACG23500.017.082984
GGTATCA153050.016.0643581
GCGCAAG23400.015.8119661
TTAACGG3400.015.77941235
AGACGGA24150.015.627336
CGGACCA23100.015.6168839
CGCAATA16550.015.53776536
TCGTTTA14750.014.92542330
TAGCGGC16850.014.821959530
ACGAACG7250.014.815
CGCGAAA1904.5656634E-1014.60526415
CGACGGT13950.014.5878147
CGTTCGG2059.276846E-1114.43902424
TATACTG17450.014.4183395
Produced by FastQC (version 0.11.5)